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mouse anti-human collagen type 2 (col2  (Developmental Studies Hybridoma Bank)


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    Developmental Studies Hybridoma Bank mouse anti-human collagen type 2 (col2
    Minimum expansion time frame to obtain chondrogenic hADSCs from the isolation phase. A Representative brightfield images of cryosections from pellet culture stained with SafraninO (in pink) and Haematoxylin (in purple) to identify cell’s nuclei. B Representative confocal images of cryosections from pellet immunostained with Phalloidin-RFP (Actin, in red), <t>Collagen</t> <t>type</t> <t>2</t> (Col 2, in cyan) and counterstained to detect cells nuclei (DAPI, in white). Superimposed channels are shown in the last row of panels (MERGE). For all the stainings, the cryosections were obtained from cells pelleted after 5 and 7 days of non-passaged proliferation, pushed into 3 weeks of chondrogenic differentiation. The calculated Collagen type 2 intensity from day 0 to day 21 in reported as Fold Increase (F.I.) in the day 21 panels of their corresponding groups
    Mouse Anti Human Collagen Type 2 (Col2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-human+collagen+type+2+(col2/collagen+type+ii+ii+ii6b3+antibody/pmc09852400-116-25-32
    Average 90 stars, based on 1 article reviews
    mouse anti-human collagen type 2 (col2 - by Bioz Stars, 2026-09
    90/100 stars

    Images

    1) Product Images from "Towards Clinical Translation of In Situ Cartilage Engineering Strategies: Optimizing the Critical Facets of a Cell-Laden Hydrogel Therapy"

    Article Title: Towards Clinical Translation of In Situ Cartilage Engineering Strategies: Optimizing the Critical Facets of a Cell-Laden Hydrogel Therapy

    Journal: Tissue Engineering and Regenerative Medicine

    doi: 10.1007/s13770-022-00487-9

    Minimum expansion time frame to obtain chondrogenic hADSCs from the isolation phase. A Representative brightfield images of cryosections from pellet culture stained with SafraninO (in pink) and Haematoxylin (in purple) to identify cell’s nuclei. B Representative confocal images of cryosections from pellet immunostained with Phalloidin-RFP (Actin, in red), Collagen type 2 (Col 2, in cyan) and counterstained to detect cells nuclei (DAPI, in white). Superimposed channels are shown in the last row of panels (MERGE). For all the stainings, the cryosections were obtained from cells pelleted after 5 and 7 days of non-passaged proliferation, pushed into 3 weeks of chondrogenic differentiation. The calculated Collagen type 2 intensity from day 0 to day 21 in reported as Fold Increase (F.I.) in the day 21 panels of their corresponding groups
    Figure Legend Snippet: Minimum expansion time frame to obtain chondrogenic hADSCs from the isolation phase. A Representative brightfield images of cryosections from pellet culture stained with SafraninO (in pink) and Haematoxylin (in purple) to identify cell’s nuclei. B Representative confocal images of cryosections from pellet immunostained with Phalloidin-RFP (Actin, in red), Collagen type 2 (Col 2, in cyan) and counterstained to detect cells nuclei (DAPI, in white). Superimposed channels are shown in the last row of panels (MERGE). For all the stainings, the cryosections were obtained from cells pelleted after 5 and 7 days of non-passaged proliferation, pushed into 3 weeks of chondrogenic differentiation. The calculated Collagen type 2 intensity from day 0 to day 21 in reported as Fold Increase (F.I.) in the day 21 panels of their corresponding groups

    Techniques Used: Isolation, Staining

    Assessment of minimal hADSCs concentration required for chondrogenesis in cell-laden hydrogel bioscaffolds. A Representative confocal images of cryosections from bioscaffolds from the 3 different hADSCs/ml groups, assessed using immunostaining for DAPI, Actin and Collagen type 2 (Col 2). The cryosections has been obtained by cutting the samples along the z axis to provide spatial information from the top to the bottom of the bioscaffolds. B Superimposed high magnification of the selected white square areas (I and II) in ( A ). C The graphs show the quantification expressed as fold change relative to 1.25 hADCSs/ml group at day 21 post chondrogenesis. The Collagen type 2 (Col 2) intensity signal was calculated and averaged from 16 different ROI from the Collagen II stained cryosections (see Figure S7). D The graphs show the quantification of Glycosaminoglycan (GAG) content measured via the normalisation of GAG over total DNA present in the processed bioscaffolds and expressed as fold change relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. E Chondrogenic gene expression analysis: the bar graphs represent the fold changes calculated with 2 ΔΔCΤ method of Collagen type 2A1 (COL2A1), Aggrecan (ACAN), Sox9 (SOX9) and Collagen type 1A2 (COL1A2) markers in RT-qPCR assay, relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. GAPDH was used as the housekeeping gene. Graph bars represent standard error margin between three biological replicates. Statistical analysis was performed using an unpaired t-test
    Figure Legend Snippet: Assessment of minimal hADSCs concentration required for chondrogenesis in cell-laden hydrogel bioscaffolds. A Representative confocal images of cryosections from bioscaffolds from the 3 different hADSCs/ml groups, assessed using immunostaining for DAPI, Actin and Collagen type 2 (Col 2). The cryosections has been obtained by cutting the samples along the z axis to provide spatial information from the top to the bottom of the bioscaffolds. B Superimposed high magnification of the selected white square areas (I and II) in ( A ). C The graphs show the quantification expressed as fold change relative to 1.25 hADCSs/ml group at day 21 post chondrogenesis. The Collagen type 2 (Col 2) intensity signal was calculated and averaged from 16 different ROI from the Collagen II stained cryosections (see Figure S7). D The graphs show the quantification of Glycosaminoglycan (GAG) content measured via the normalisation of GAG over total DNA present in the processed bioscaffolds and expressed as fold change relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. E Chondrogenic gene expression analysis: the bar graphs represent the fold changes calculated with 2 ΔΔCΤ method of Collagen type 2A1 (COL2A1), Aggrecan (ACAN), Sox9 (SOX9) and Collagen type 1A2 (COL1A2) markers in RT-qPCR assay, relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. GAPDH was used as the housekeeping gene. Graph bars represent standard error margin between three biological replicates. Statistical analysis was performed using an unpaired t-test

    Techniques Used: Concentration Assay, Immunostaining, Staining, Expressing, Quantitative RT-PCR

    In situ stem cells-laden hydrogel therapy in a rabbit in vivo cartilage repair model. A Representative macroscopic pictures (Macro) and images from Haematoxylin and Eosin (H&E) stained paraffin sections from explanted samples of the indicated groups. Representative confocal images of paraffin sections from explanted samples of the indicated groups assessed using immunostaining for Collagen type 2 (Col 2, in cyan) and Collagen type 1 (Col 1, in red). Overimposed images of the two channels are shown in the Merge raw. B The graph shows the macroscopic score using the International Cartilage Repair Society (ICRS) system for the indicated groups, calculated at the end of the 8 weeks study on the explants. C The graph shows the microscopic score calculated at the end of the 8 weeks study on the HandE and Col1 and 2 stained paraffin sections. D The graph shows the percentage of the Collagen 1 (Col 1) and Collagen 2 (Col 2) positive areas. Graph bars represents the mean with standard deviation of 4 different regions along the entire diameter of the defect for each sample analysed calculated at the end of the 8 weeks study on the immunostained paraffin sections. E The graph shows the biomechanical evaluation using atomic force microscopy calculated at the end of the 8 weeks study on the explants and expressed as Young Modulus (kPa kilopascals). Statistical analysis was performed using unpaired t-test
    Figure Legend Snippet: In situ stem cells-laden hydrogel therapy in a rabbit in vivo cartilage repair model. A Representative macroscopic pictures (Macro) and images from Haematoxylin and Eosin (H&E) stained paraffin sections from explanted samples of the indicated groups. Representative confocal images of paraffin sections from explanted samples of the indicated groups assessed using immunostaining for Collagen type 2 (Col 2, in cyan) and Collagen type 1 (Col 1, in red). Overimposed images of the two channels are shown in the Merge raw. B The graph shows the macroscopic score using the International Cartilage Repair Society (ICRS) system for the indicated groups, calculated at the end of the 8 weeks study on the explants. C The graph shows the microscopic score calculated at the end of the 8 weeks study on the HandE and Col1 and 2 stained paraffin sections. D The graph shows the percentage of the Collagen 1 (Col 1) and Collagen 2 (Col 2) positive areas. Graph bars represents the mean with standard deviation of 4 different regions along the entire diameter of the defect for each sample analysed calculated at the end of the 8 weeks study on the immunostained paraffin sections. E The graph shows the biomechanical evaluation using atomic force microscopy calculated at the end of the 8 weeks study on the explants and expressed as Young Modulus (kPa kilopascals). Statistical analysis was performed using unpaired t-test

    Techniques Used: In Situ, In Vivo, Staining, Immunostaining, Standard Deviation, Microscopy

    Related Articles

    Incubation:

    Article Title: Mesenchymal progenitor cells from non-inflamed versus inflamed synovium post-ACL injury present with distinct phenotypes and cartilage regeneration capacity.
    Article Snippet: .. Human nuclear antigen (HNA; Clone # 235-1, Abcam) or Collagen 2 (Col2; Clone # II-II6B3, DSHB) was applied to the sections and incubated overnight. .. For the Col2 staining an additional hyaluronidase (Sigma) treatment step was added.

    Article Title: Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models
    Article Snippet: .. After treatment with horse serum for 1 h, sections were incubated with primary antibodies against COL2 (DSHB, CIICI; 2 μg/mL), and ACAN (DSHB, 1C6; 5 μg/mL) for 30 min at room temperature, successively followed by binding with biotinylated horse anti-mouse secondary antibodies (Vector laboratories, BA-2001; 1:200) for 30 min, ABC-complex (Vector laboratories) for 30 min, and DAB (Vector laboratories) for 4 min. ..

    Article Title: Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of osteoarthritis
    Article Snippet: .. Antigen retrieval was achieved using 10 mM sodium citrate (pH 6.0), and non-specific blocking was prevented using goat serum (1:500 dilution in TBST). tdTomato (AB8181, Origene), Collagen 2 (Col2; Clone # II-II6B3, DSHB), PRG4 (Clone # 9G3, Millipore), Ccl2 (Clone # 2D8, Thermo Fisher), Ki67 (Clone # SolA15, ThermoFisher), Sox9 (Clone # 7H13L8, Thermo Fisher) or CD47 (Clone # B6H12, Thermo Fisher) were applied to the sections and incubated overnight. .. For the Col2 staining, an additional hyaluronidase (Sigma) treatment step was added.

    Article Title: Mesenchymal progenitor cells from non-inflamed versus inflamed synovium post-ACL injury present with distinct phenotypes and cartilage regeneration capacity
    Article Snippet: .. Human nuclear antigen (HNA; Clone # 235-1, Abcam) or Collagen 2 (Col2; Clone # II-II6B3, DSHB) was applied to the sections and incubated overnight. .. For the Col2 staining an additional hyaluronidase (Sigma) treatment step was added.

    Article Title: Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models.
    Article Snippet: .. After treatment with horse serum for 1 h, sections were incubated with primary antibodies against COL2 (DSHB, CIICI; 2 μg/mL), and ACAN (DSHB, 1C6; 5 μg/mL) for 30 min at room temperature, successively followed by binding with biotinylated horse anti-mouse secondary antibodies (Vector laboratories, BA-2001; 1:200) for 30 min, ABC-complex (Vector laboratories) for 30 min, and DAB (Vector laboratories) for 4 min. ..

    other:

    Article Title: Towards Clinical Translation of In Situ Cartilage Engineering Strategies: Optimizing the Critical Facets of a Cell-Laden Hydrogel Therapy
    Article Snippet: After washing, samples were dropped in blocking solution (10% goat serum diluted in PBT) for 60 min and then incubated overnight at 4 °C with mouse anti-human Collagen type 2 (Col2) (#II6B3, DSHB) and goat anti-human Collagen type 1 (Col1) (# sc8784, Santa Cruz Biotechnology).

    Binding Assay:

    Article Title: Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models
    Article Snippet: .. After treatment with horse serum for 1 h, sections were incubated with primary antibodies against COL2 (DSHB, CIICI; 2 μg/mL), and ACAN (DSHB, 1C6; 5 μg/mL) for 30 min at room temperature, successively followed by binding with biotinylated horse anti-mouse secondary antibodies (Vector laboratories, BA-2001; 1:200) for 30 min, ABC-complex (Vector laboratories) for 30 min, and DAB (Vector laboratories) for 4 min. ..

    Article Title: Anti-inflammatory and pro-anabolic effects of 5-aminosalicylic acid on human inflammatory osteoarthritis models.
    Article Snippet: .. After treatment with horse serum for 1 h, sections were incubated with primary antibodies against COL2 (DSHB, CIICI; 2 μg/mL), and ACAN (DSHB, 1C6; 5 μg/mL) for 30 min at room temperature, successively followed by binding with biotinylated horse anti-mouse secondary antibodies (Vector laboratories, BA-2001; 1:200) for 30 min, ABC-complex (Vector laboratories) for 30 min, and DAB (Vector laboratories) for 4 min. ..

    Blocking Assay:

    Article Title: Identification of a sub-population of synovial mesenchymal stem cells with enhanced treatment efficacy in a rat model of osteoarthritis
    Article Snippet: .. Antigen retrieval was achieved using 10 mM sodium citrate (pH 6.0), and non-specific blocking was prevented using goat serum (1:500 dilution in TBST). tdTomato (AB8181, Origene), Collagen 2 (Col2; Clone # II-II6B3, DSHB), PRG4 (Clone # 9G3, Millipore), Ccl2 (Clone # 2D8, Thermo Fisher), Ki67 (Clone # SolA15, ThermoFisher), Sox9 (Clone # 7H13L8, Thermo Fisher) or CD47 (Clone # B6H12, Thermo Fisher) were applied to the sections and incubated overnight. .. For the Col2 staining, an additional hyaluronidase (Sigma) treatment step was added.



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    mouse anti-human collagen type 2 (col2  (Developmental Studies Hybridoma Bank)
    90
    Developmental Studies Hybridoma Bank mouse anti-human collagen type 2 (col2
    Minimum expansion time frame to obtain chondrogenic hADSCs from the isolation phase. A Representative brightfield images of cryosections from pellet culture stained with SafraninO (in pink) and Haematoxylin (in purple) to identify cell’s nuclei. B Representative confocal images of cryosections from pellet immunostained with Phalloidin-RFP (Actin, in red), <t>Collagen</t> <t>type</t> <t>2</t> (Col 2, in cyan) and counterstained to detect cells nuclei (DAPI, in white). Superimposed channels are shown in the last row of panels (MERGE). For all the stainings, the cryosections were obtained from cells pelleted after 5 and 7 days of non-passaged proliferation, pushed into 3 weeks of chondrogenic differentiation. The calculated Collagen type 2 intensity from day 0 to day 21 in reported as Fold Increase (F.I.) in the day 21 panels of their corresponding groups
    Mouse Anti Human Collagen Type 2 (Col2, supplied by Developmental Studies Hybridoma Bank, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/mouse+anti-human+collagen+type+2+(col2/collagen+type+ii+ii+ii6b3+antibody/pmc09852400-116-25-32
    Average 90 stars, based on 1 article reviews
    mouse anti-human collagen type 2 (col2 - by Bioz Stars, 2026-09
    90/100 stars
    		  Buy from Supplier

    Image Search Results


    Minimum expansion time frame to obtain chondrogenic hADSCs from the isolation phase. A Representative brightfield images of cryosections from pellet culture stained with SafraninO (in pink) and Haematoxylin (in purple) to identify cell’s nuclei. B Representative confocal images of cryosections from pellet immunostained with Phalloidin-RFP (Actin, in red), Collagen type 2 (Col 2, in cyan) and counterstained to detect cells nuclei (DAPI, in white). Superimposed channels are shown in the last row of panels (MERGE). For all the stainings, the cryosections were obtained from cells pelleted after 5 and 7 days of non-passaged proliferation, pushed into 3 weeks of chondrogenic differentiation. The calculated Collagen type 2 intensity from day 0 to day 21 in reported as Fold Increase (F.I.) in the day 21 panels of their corresponding groups

    Journal: Tissue Engineering and Regenerative Medicine

    Article Title: Towards Clinical Translation of In Situ Cartilage Engineering Strategies: Optimizing the Critical Facets of a Cell-Laden Hydrogel Therapy

    doi: 10.1007/s13770-022-00487-9

    Figure Lengend Snippet: Minimum expansion time frame to obtain chondrogenic hADSCs from the isolation phase. A Representative brightfield images of cryosections from pellet culture stained with SafraninO (in pink) and Haematoxylin (in purple) to identify cell’s nuclei. B Representative confocal images of cryosections from pellet immunostained with Phalloidin-RFP (Actin, in red), Collagen type 2 (Col 2, in cyan) and counterstained to detect cells nuclei (DAPI, in white). Superimposed channels are shown in the last row of panels (MERGE). For all the stainings, the cryosections were obtained from cells pelleted after 5 and 7 days of non-passaged proliferation, pushed into 3 weeks of chondrogenic differentiation. The calculated Collagen type 2 intensity from day 0 to day 21 in reported as Fold Increase (F.I.) in the day 21 panels of their corresponding groups

    Article Snippet: After washing, samples were dropped in blocking solution (10% goat serum diluted in PBT) for 60 min and then incubated overnight at 4 °C with mouse anti-human Collagen type 2 (Col2) (#II6B3, DSHB) and goat anti-human Collagen type 1 (Col1) (# sc8784, Santa Cruz Biotechnology).

    Techniques: Isolation, Staining

    Assessment of minimal hADSCs concentration required for chondrogenesis in cell-laden hydrogel bioscaffolds. A Representative confocal images of cryosections from bioscaffolds from the 3 different hADSCs/ml groups, assessed using immunostaining for DAPI, Actin and Collagen type 2 (Col 2). The cryosections has been obtained by cutting the samples along the z axis to provide spatial information from the top to the bottom of the bioscaffolds. B Superimposed high magnification of the selected white square areas (I and II) in ( A ). C The graphs show the quantification expressed as fold change relative to 1.25 hADCSs/ml group at day 21 post chondrogenesis. The Collagen type 2 (Col 2) intensity signal was calculated and averaged from 16 different ROI from the Collagen II stained cryosections (see Figure S7). D The graphs show the quantification of Glycosaminoglycan (GAG) content measured via the normalisation of GAG over total DNA present in the processed bioscaffolds and expressed as fold change relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. E Chondrogenic gene expression analysis: the bar graphs represent the fold changes calculated with 2 ΔΔCΤ method of Collagen type 2A1 (COL2A1), Aggrecan (ACAN), Sox9 (SOX9) and Collagen type 1A2 (COL1A2) markers in RT-qPCR assay, relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. GAPDH was used as the housekeeping gene. Graph bars represent standard error margin between three biological replicates. Statistical analysis was performed using an unpaired t-test

    Journal: Tissue Engineering and Regenerative Medicine

    Article Title: Towards Clinical Translation of In Situ Cartilage Engineering Strategies: Optimizing the Critical Facets of a Cell-Laden Hydrogel Therapy

    doi: 10.1007/s13770-022-00487-9

    Figure Lengend Snippet: Assessment of minimal hADSCs concentration required for chondrogenesis in cell-laden hydrogel bioscaffolds. A Representative confocal images of cryosections from bioscaffolds from the 3 different hADSCs/ml groups, assessed using immunostaining for DAPI, Actin and Collagen type 2 (Col 2). The cryosections has been obtained by cutting the samples along the z axis to provide spatial information from the top to the bottom of the bioscaffolds. B Superimposed high magnification of the selected white square areas (I and II) in ( A ). C The graphs show the quantification expressed as fold change relative to 1.25 hADCSs/ml group at day 21 post chondrogenesis. The Collagen type 2 (Col 2) intensity signal was calculated and averaged from 16 different ROI from the Collagen II stained cryosections (see Figure S7). D The graphs show the quantification of Glycosaminoglycan (GAG) content measured via the normalisation of GAG over total DNA present in the processed bioscaffolds and expressed as fold change relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. E Chondrogenic gene expression analysis: the bar graphs represent the fold changes calculated with 2 ΔΔCΤ method of Collagen type 2A1 (COL2A1), Aggrecan (ACAN), Sox9 (SOX9) and Collagen type 1A2 (COL1A2) markers in RT-qPCR assay, relative to 1.25 hADSCs/ml group at day 21 post chondrogenesis. GAPDH was used as the housekeeping gene. Graph bars represent standard error margin between three biological replicates. Statistical analysis was performed using an unpaired t-test

    Article Snippet: After washing, samples were dropped in blocking solution (10% goat serum diluted in PBT) for 60 min and then incubated overnight at 4 °C with mouse anti-human Collagen type 2 (Col2) (#II6B3, DSHB) and goat anti-human Collagen type 1 (Col1) (# sc8784, Santa Cruz Biotechnology).

    Techniques: Concentration Assay, Immunostaining, Staining, Expressing, Quantitative RT-PCR

    In situ stem cells-laden hydrogel therapy in a rabbit in vivo cartilage repair model. A Representative macroscopic pictures (Macro) and images from Haematoxylin and Eosin (H&E) stained paraffin sections from explanted samples of the indicated groups. Representative confocal images of paraffin sections from explanted samples of the indicated groups assessed using immunostaining for Collagen type 2 (Col 2, in cyan) and Collagen type 1 (Col 1, in red). Overimposed images of the two channels are shown in the Merge raw. B The graph shows the macroscopic score using the International Cartilage Repair Society (ICRS) system for the indicated groups, calculated at the end of the 8 weeks study on the explants. C The graph shows the microscopic score calculated at the end of the 8 weeks study on the HandE and Col1 and 2 stained paraffin sections. D The graph shows the percentage of the Collagen 1 (Col 1) and Collagen 2 (Col 2) positive areas. Graph bars represents the mean with standard deviation of 4 different regions along the entire diameter of the defect for each sample analysed calculated at the end of the 8 weeks study on the immunostained paraffin sections. E The graph shows the biomechanical evaluation using atomic force microscopy calculated at the end of the 8 weeks study on the explants and expressed as Young Modulus (kPa kilopascals). Statistical analysis was performed using unpaired t-test

    Journal: Tissue Engineering and Regenerative Medicine

    Article Title: Towards Clinical Translation of In Situ Cartilage Engineering Strategies: Optimizing the Critical Facets of a Cell-Laden Hydrogel Therapy

    doi: 10.1007/s13770-022-00487-9

    Figure Lengend Snippet: In situ stem cells-laden hydrogel therapy in a rabbit in vivo cartilage repair model. A Representative macroscopic pictures (Macro) and images from Haematoxylin and Eosin (H&E) stained paraffin sections from explanted samples of the indicated groups. Representative confocal images of paraffin sections from explanted samples of the indicated groups assessed using immunostaining for Collagen type 2 (Col 2, in cyan) and Collagen type 1 (Col 1, in red). Overimposed images of the two channels are shown in the Merge raw. B The graph shows the macroscopic score using the International Cartilage Repair Society (ICRS) system for the indicated groups, calculated at the end of the 8 weeks study on the explants. C The graph shows the microscopic score calculated at the end of the 8 weeks study on the HandE and Col1 and 2 stained paraffin sections. D The graph shows the percentage of the Collagen 1 (Col 1) and Collagen 2 (Col 2) positive areas. Graph bars represents the mean with standard deviation of 4 different regions along the entire diameter of the defect for each sample analysed calculated at the end of the 8 weeks study on the immunostained paraffin sections. E The graph shows the biomechanical evaluation using atomic force microscopy calculated at the end of the 8 weeks study on the explants and expressed as Young Modulus (kPa kilopascals). Statistical analysis was performed using unpaired t-test

    Article Snippet: After washing, samples were dropped in blocking solution (10% goat serum diluted in PBT) for 60 min and then incubated overnight at 4 °C with mouse anti-human Collagen type 2 (Col2) (#II6B3, DSHB) and goat anti-human Collagen type 1 (Col1) (# sc8784, Santa Cruz Biotechnology).

    Techniques: In Situ, In Vivo, Staining, Immunostaining, Standard Deviation, Microscopy